| GeneLibs

题目:: Transcription profiling of inhibitor 1 knock out mice vs controls to examine the role of inhibitor-1/PP1 escape from cellular homeostasis and regulation of neuronal learning and memory
ID:
状态:: 发布时间July 7, 2007 , 更新时间 June 10, 2011 , 提交时间 Jan. 13, 2006,
物种:: Mus musculus
摘要:: Reversible phosphorylation is a critical step in the control of cellular signaling events. The protein phosphatase 1 (PP1) regulates neuronal proteins by mediating their de-phosphorylation. PP1 selectivity and subcellular localization is conferred via association with a panel of interacting proteins. We have extensively characterized the association of PP1 with inhibitor-1, an interacting protein widely expressed in brain and heart. Inhibitor-1 deletion results in a persistently active PP1 phosphatase in vitro. Inhibitor-1 overexpression results in increased transformation of cultured cells and enhances the rate of learning and memory in mouse models through largely unknown mechanisms. Significantly, inhibitor-1 activation is cAMP-sensitive. The genes regulated by inhibitor-1/PP1 signals have not been characterized. Identification of these genes is expected to provide key insights into the role of inhibitor-1/PP1 escape from cellular homeostasis and regulation of neuronal learning and memory.,We will determine gene expression patterns in hippocampus of wild-type and inhibitor-1 knock-out mice. The results of these studies of constitutive expression will be used as a baseline for future studies aimed to determine the role cAMP-mediated gene expression in I-1 knock-out mice.,We hypothesize that the genes regulated by the inhibitor-1/PP1 holoenzyme are critical to cellular growth commitment including resistance to growth arrest and neuronal learning and memory,We will examine the role of inhibitor-1 in regulating hippocampal gene expression using total RNA isolated from whole hippocampi of inhibitor-1 knockout (I-1-/-) animals and parental C57Bl6 controls under non-stimulated conditions. Animals will be sacrificed by a standard protocol. Hippocampal tissue will be rapidly dissected from several animals, flash frozen within 1 minute of extraction, pooled, and total RNA will be processed immediately using TriZol.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-4040, GSE4040, GDS1920
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息

DataTable pData

联系方式

微信公众号

实验库 数据相关信息

DataTable pData

联系方式

微信公众号

实验库数据相关信息