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题目:: Beta-naphthoflavone treated worms
ID:
状态:: 发布时间Nov. 25, 2005 , 更新时间 March 27, 2012 , 提交时间 Nov. 25, 2005,
物种:: Caenorhabditis elegans
摘要:: The soil nematode Caenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its genome contains 80 cytochrome P450 genes (CYP). In order to study CYP gene expression in C. elegans mixed stages and synchronized hermaphrodites were exposed to 18 known xenobiotic cytochrome P450 inducers. Messenger RNA expression was detected by DNA arrays and semiquantitative RT-PCR. Using subfamily-specific primers, a pooled set of exon-rich CYP fragments could be amplified. In this way it was possible to systematically check the influence of different inducers on CYP expression at the same time. The well-known CYP1A inducers beta-naphthoflavone, PCB52, and lansoprazol were the most active and in particular they strongly induced almost all CYP35 isoforms. A few number of further CYP forms were found to be inducible by other xenobiotics like phenobarbital, atrazine, and clofibrate. In addition, a transgenic C. elegans line expressing GFP under control of the CYP35A2 promoter showed a strong induction of the fusion by beta-naphthoflavone in the intestine. Copyright 2001 Academic Press. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
实验种类:: unknown experiment type
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-3680, GSE3680
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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