Pyrococcus furiosus crRNAs associated with the Cas RAMP module complex

·发布 2012年1月31日 ·更新 2012年11月6日

样本数

实验数

相关文献

实验描述

Analysis of CRISPR RNAs from both total Pyrococcus furiosus RNA and those that co-purify with the Cmr complex, which has been predicted to be involved in RNA-mediated targeting of foreign RNAs in prokaryotes. Small RNAs (20 - 70 nts) were isolated either from total Pyrococcus furiosus RNA or RNAs isolated from immunopurified Cmr complexes. The RNAs were cloned and sequenced using the Illumina platform. Results provide insight into the anatomy of Cmr2-associated CRISPR RNAs. 2 Samples: total small RNAs vs Cmr2-associated small RNAs

参考文献

Essential features and rational design of CRISPR RNAs that function with the Cas RAMP module complex to cleave RNAs.

Hale CR, Majumdar S, Elmore J, Pfister N, Compton M, Olson S, Resch AM, Glover CV 3rd, Graveley BR, Terns RM, Terns MP

PMID: 22227116

样本属性

cell

Cultured organisms

Organism

Pyrococcus furiosus

strain or line

DSM 3638

实验信息

登记号

E-GEOD-33256

GEO 编号

GSE33256, SRP009098

实验类型

RNA-seq of coding RNA

物种

Pyrococcus furiosus

发布日期

2012年1月31日

更新日期

2012年11月6日

提交者

Mark Compton、 Sara Olson、 Alissas M Resch、 Sonali Majumdar、 Rebecca Terns、 Caryn R Hale、 Neil Pfister、 Claiborne V Glover III、 Joshua Elmore、 Brenton R Graveley、 Michael Terns

外部链接

ArrayExpress 原始页 NCBI GEO：GSE33256, SRP009098

分析服务

Pyrococcus furiosus crRNAs associated with the Cas RAMP module complex

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