Transcription profiling of different types of cells in murine hair follicle to study cell type-specific gene expression
ID:
状态:
发布时间Sept. 7, 2007
, 更新时间 March 27, 2012
, 提交时间 Aug. 16, 2005,
物种:
Mus musculus
摘要:
Hair follicle matrix, outer root sheath, dermal papilla cells and melanocytes and a dermal fraction enriched in fibroblasts were FACS isolated from 4d backskins. Targets from two biological replicates of each were generated and the expression profiles were determined using Affymetrix Mouse Genechip 430A arrays. Comparisons between the sample groups allow the identification of cell-type specific genes. Experiment Overall Design: Mx, ORS, DF, DP and Mc were FACSorted from Lef1-RFP/K14-H2BGFP mice from 4 day old backskins