Transcription profiling of human Hematopoietic stem cell (HSC) untreated and treated with UTP 10uM at 1, 6 and 24
ID:
状态:
发布时间June 12, 2008
, 更新时间 June 10, 2011
, 提交时间 July 26, 2005,
物种:
Homo sapiens
摘要:
Hematopoietic stem cell (HSC) untreated and treated with UTP 10uM at 1, 6 and 24 h Experiment Overall Design: Highly purified CD34+ cells from 6 healthy donors were seeded at 1000000 cells/ml in serum free medium (EX vivo 15) w/o cytokines and treated with 10 uM UTP for 1, 6 and 24 hours. As a control, CD34+ untreated cells were maintened in the same culture conditions for the same times. RNAs originating from the 6 donors were pooled in order to obtain at least 2 mg per sample (untreated and UTP-treated respectively).