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题目:: Transcription profiling of Arabidopsis dark grown photoreceptor mutants seedlings
ID:
状态:: 发布时间June 12, 2008 , 更新时间 March 27, 2012 , 提交时间 July 26, 2005,
物种:: Arabidopsis thaliana
摘要:: Phytochromes mediate a profound developmental shift when dark-grown seedlings are exposed to light. Here we show that a subset of genes is up regulated in phytochrome B (phyB) mutants even before dark-grown seedlings are exposed to light. Most of these genes bear the RY cis motif, which is a binding site of the transcription factor ABSCISIC ACID INSENSITIVE 3 (ABI3), and the phyB mutation also enhanced ABI3 expression. These changes in transcriptome have physiological consequences as seedlings of the abi3 mutant showed enhanced responses to pulses of far-red light, while ABI3 overexpressers exhibited the opposite pattern. Seedlings of the wild type derived from seeds germinated in full darkness showed enhanced expression of genes bearing the RY cis motif and reduced responses to far-red light. We propose that, via changes in ABI3 expression, light, perceived mainly by phyB in the seed, generates a downstream trans-developmental phase signal that pre-conditions the seedling to its most likely environment. Experiment Overall Design: We analysed the transcriptome profile in dark grown seedlings of the WT, and phyA phyB photoreceptor mutants. All seedlings have the same treatment (3d grown in complete darkness). We have two samples for each genotype that represent two replicates in each case.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-GEOD-3002, GDS1702, GSE3002
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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