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题目:: Transcription profiling of synovial mouse fibroblast activated by IL-1beta effect of prostacyclin signaling
ID:
状态:: 发布时间June 12, 2008 , 更新时间 March 27, 2012 , 提交时间 May 18, 2005,
物种:: Mus musculus
摘要:: Synovial fibroblasts were extracted from knee joints of naïve mice(DBA/1J, male 8-12 week old), and passaged 3-4 times. Cells were plated at a concentration of 1 X 106 cells/dish in 60 mm dish in 5 ml of RPMI1640 medium with 1% heat-inactivated FCS at 37°C, 5% CO2. Cells were treated vehicle or stimulated by IL-1beta. In IL-1beta(5ng/ml) stimulated groups, indomethacin (1 μM) and iloprost (1μM) were further added either alone or in combination. After 6 hour stimulation as described above, cells were isolated and frozen by liquid nitrogen, and stored at –80°C before use. Total RNA was extracted by RNAeasy Mini Kit. The RNA sample was labeled for hybridization onto the genechip array according to the standard affymetrix protcols.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-GEOD-2676, GSE2676, GDS1796
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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