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题目:: Transcriptome effects of silencing a MAPK (SIPK) in tobacco
ID:
状态:: 发布时间June 10, 2010 , 更新时间 Oct. 18, 2011 , 提交时间 May 16, 2005,
物种:: Nicotiana tabacum
摘要:: Wildtype and SIPK -silenced (RNAi) tobacco cell suspension cultures were grown in MS medium at 25 C in the dark. Cell suspension cultures were transferred to fresh growth medium 3 days prior to pooling each genotype and dividing the pool into 3 x 10ml volumes per genotype. A 30 minute pre-incubation was used with xanthine (for abiotic controls and treatments), but no pre-treatment was used for the biotic stressors. For each treatment and abiotic/biotic controls, the cells were harvested by vacuum filtration at the appropriate time points (controls sampled at 0 hrs) and frozen in N2(l) for RNA extraction. In order to discern the effect of silencing SIPK on the transciptome in defence response, biological replicates of WT and SIPK-Ri were randomly paired on 3 slides per treatment or control group. Series_weblink:
实验种类:: unknown experiment type
样本量:: 64
实验设计:
: 无设计数据
数据号:: E-GEOD-2675, GSE2675
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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