实验库 数据相关信息
- 题目:
- Whole Transcriptome Amplification for Gene Expression Profiling and Development of Molecular Archives
- ID:
- 状态:
- 发布时间June 11, 2010 , 更新时间 March 27, 2012 , 提交时间 May 3, 2005,
- 物种:
- Homo sapiens
- 摘要:
- Type of experiment: This study used microarray experiments to evaluate the reproducibility and fidelity of Whole Transcriptome Amplification (WTA). Experimental factors: This study contains multiple categories of microarray experiments. Self-self hybridizations from WTA amplified total RNA were performed to evaluate the reproducibility of WTA. To evaluate the fidelity of WTA, the same total RNA, isolated from tissue samples or cell lines, was either directly used for microarray analysis or subjected to WTA amplification before hybridization. The amount of input total RNA for WTA amplification was varied for different hybridizations. To demonstrate the usefulness of WTA, benign epithelia, cancerous epithelia and stroma isolated by laser capture microdissection from frozen prostate tissue specimens were subjected to WTA and hybridized. To demonstrate the fidelity of WTA with degraded samples, total RNA from cell lines was artificially degraded before WTA and hybridization. Total RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissues containing benign and cancerous prostate tissue were also subjected to WTA and hybridized.
- 实验种类:
- unknown experiment type
- 样本量:
- 82
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-2618, GSE2618
- 数据状态:
无法自动分析，您可以尝试手动分析数据。
DataTable pData
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