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题目:: Rice Seedling Stress treatment
ID:
状态:: 发布时间June 11, 2010 , 更新时间 Oct. 18, 2011 , 提交时间 March 17, 2005,
物种:: Oryza sativa
摘要:: The japonica rice variety Nipponbare was grown under hydroponic condition for 10-days (untreated seedling sample). Seedlings were exposed to once stress conditions for 24 hours (stress treated sample). Seedlings were exposed to one of the following for 24 h: cold stress, incubation at 10 °C; salt stress by adding 150 mM sodium chloride to the planter box; osmotic stress by adding 260 mM mannitol to the planter box; and drought stress by adding 25% polyethylene glycol 6000 to the planter box. Total RNAs were prepared from each sample using an RNeasy Midi Kit (Qiagen, Tokyo, Japan) and the mRNAs were purified with an Oligotex-dT30 Kit (Takara, Shiga, Japan). Purified mRNA was amplified and labeled and we hybridized the 1-mg fluorescent liner amplified Cy3- and Cy5-labeled cRNAs (each cRNA was 500 ng) to the NIAS RICE 22K oligonucleotide array ver1 according to the manufacturer. Keywords = Rice Keywords = Seedling Keywords = stress treatment Keywords = flood
实验种类:: unknown experiment type
样本量:: 24
实验设计:
: 无设计数据
数据号:: E-GEOD-2415, GSE2415
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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