Systemic inflammation modulates Fc receptor expression on microglia in chronic neurodegeneration
ID:
状态:
发布时间July 28, 2010
, 更新时间 June 10, 2011
物种:
Mus musculus
摘要:
To investigate the inflammatory response in neurodegenerative disease during an episode of systemic inflammation using the ME7 prion model. Comparison of ME7-infected mice with mice innoculated with Normal brain homogenate (NBH). 18 weeks post-innoculation mice were administered 500ug/kg LPS or an equivalent volume of saline as a control. Total RNA was extracted from the hippocampus, amplified and reverse transcribed to cDNA using WT ovation kit and hybridised to mouse 430_2.0 Affymetrix Arrays. There were three biological replicates (individual mice) per group.