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题目:: Saccharomyces cerevisiae grown in nitrogen depletion with MTP priming
ID:
状态:: 发布时间July 17, 2010 , 更新时间 May 1, 2014
物种:: Saccharomyces cerevisiae
摘要:: Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Saccharomyces cerevisiae S288c was grown on two different media.
实验种类:: transcription profiling by array
样本量:: 8
实验设计:
: 无设计数据
数据号:: E-GEOD-22936, GSE22936
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