实验库 数据相关信息

题目:
FnrL regulon in Rhodobacter sphaeroides
ID:
状态:
发布时间July 1, 2010 , 更新时间 May 2, 2014
物种:
Rhodobacter sphaeroides 2.4.1
摘要:
In this study, we performed a ChIP-chip experiment to determine the regulon of FnrL in Rhodobacter sphaeroides. We grew R. sphaeroides under anaerobic photosnthetic conditions, in which FnrL is expected to bind DNA and contol gene expression, and immuno-precipitated FnrL, but also sigma70 and the Beta' subunits of RNA polymerase to determine transcription activity. DNA immunoprecipitated with polyclonal antibodies against FnrL, sigma70, or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.
实验种类:
ChIP-chip by tiling array
样本量:
18
实验设计:
无设计数据
数据号:
E-GEOD-22027, GSE22027
数据状态:

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