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题目:: Expression analysis of Naegleria gruberi (strain NEG) during differentiation from the amoeba to the flagellate form
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状态:: 发布时间Sept. 2, 2010 , 更新时间 June 10, 2011
物种:: Naegleria gruberi
摘要:: Naegleria gruberi is a single-celled eukaryote best known for its remarkable ability to form an entire microtubule cytoskeleton de novo during its metamorphosis from an amoeba into a flagellate, including two basal bodies (equivalent to centrioles), two flagella (equivalent to cilia), and a cytoplasmic microtubule array. This full-genome transcriptional analysis, performed at 20-minute intervals throughout Naegleria differentiation, reveals vast transcriptional changes, including the differential expression of cytoskeletal, metabolism, signaling and stress response genes. Naegleria gruberi (strain NEG) was grown in association with Kleibsiella pneumoniae on solid media. Cells were prepared and differentiated using standard protocols, and harvested at 0, 20, 40, 60 and 80 minutes after initiation of differentiation. Three independent biological replicates were obtained from differentiating N. gruberi. Each replicate series included the following timepoints: 0, 20, 40, 60 and 80 minutes after initiation of differentiation.
实验种类:: transcription profiling by array
样本量:: 15
实验设计:
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数据号:: E-GEOD-21527, GSE21527
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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