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题目:: Expression profile of TTHA1634-deficient Thermus thermophilus HB8 strain
ID:
状态:: 发布时间Oct. 15, 2010 , 更新时间 June 10, 2011 , 提交时间 April 21, 2010,
物种:: Thermus thermophilus HB8
摘要:: In order to find function of TTHA1634 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1634-deficient strain grown in a rich medium at 70°C for 420, and 600 min. Two TTHA1634-deficient T. thermophilus HB8 strains were each pre-cultured at 70°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70°C. Cells were collected after 420, and 600 min, respectively, and then, the expression of each mRNA was analyzed.
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-GEOD-21460, GSE21460
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