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题目:: Development of the Restriction Site Tiling Analysis (RSTA) method: Accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
ID:
状态:: 发布时间March 12, 2010 , 更新时间 May 2, 2014 , 提交时间 March 12, 2010,
物种:: Strongylocentrotus purpuratus
摘要:: Here we developed a new high-throughput polymorphism detection and genotyping method based on identifying restriction cut site polymorphisms using a microarray platform. We compared the genomes of 20 individual urchins; 10 from the northern part of the species range (Boiler Bay, OR) and 10 from the southern part of the range (San Diego, CA). Genomic DNA from each individual was extracted, randomly sheared, then divided in half. Half was restriction digested and labeled with Cy3, half was treated as control and labeled with Cy5. Labeled DNA from a single individual was then competitively hybridized to the custom, species-specific array that had probe sequences designed to be centered on known restriction cut sites. Resulting log ratio data reflected the three possible genotypes in clusters when arrays from multiple individuals were examined at a given polymorphic locus.
实验种类:: comparative genomic hybridization by array
样本量:: 40
实验设计:
: 无设计数据
数据号:: E-GEOD-20857, GSE20857
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