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题目:: Steady State Thermal Acclimation in the Eurythermal Goby fish, Gillichthys mirabilis
ID:
状态:: 发布时间July 15, 2010 , 更新时间 May 2, 2014
物种:: Gillichthys mirabilis
摘要:: The goal of this study was to identify transcriptional resonses to thermal acclimation in a highly eurythermal fish. Specifically, what are the genes necessary to maintain homostasis at different temperatures and do these genes underlie any known acclimatory responses? Gillichthys mirabilis (goby fish) were acclimated to 3 temperatures (9C, 19C and 28C) for one month. Gill tissue from 7-9 individuals were dissected and immediately flash frozen in liquid nitrogen. Total RNA was extracted, reverse transcribed into cDNA, labeled with cy5 and hybridized against a common reference (cy3) on 2-color cDNA microarray developed for this species. Significant genes were determined by expression differences between groups (one-way ANOVA, FDR<0.05) . Common reference design
实验种类:: transcription profiling by array
样本量:: 44
实验设计:
: 无设计数据
数据号:: E-GEOD-20849, GSE20849
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