实验库 数据相关信息
- 题目:
- Nuclear gene expression in response to chloroplast division inhibition
- ID:
- 状态:
- 发布时间April 28, 2010 , 更新时间 May 1, 2014 , 提交时间 Feb. 8, 2010,
- 物种:
- Arabidopsis thaliana
- 摘要:
- The aim of this investigation is to analyse the effect on nuclear gene expression of inhibition of chloroplast division. Transgenic Arabidopsis lines (Ler) were generated that contained a chemically inducible promoter (XVE) upstream of AtMinD1. AtMinD1 encodes a product involved in chloroplast division; overexpression of AtMinD1 leads to chloroplast division inhibition. 6 samples were used in this experiment. Three treatments were performed: Treatment 1: Empty vector control seedlings (transformed with PER-10 only) were sown onto lehle, sprayed at 13 days old with 2uM 17-B-estradiol (inducer) and harvested 24 hours later. Treatment 2: Seeds were sown on lehle containing inducer and sprayed every 24 hours with 2uM inducer until harvested at 14 days old. Treatment 3: Seeds were sown on lehle plates, sprayed at 13 days old with 2uM inducer and harvested 24 hours later. For all three treatments, seedlings were grown in constant light conditions, and tissue was harvested from 14-day-old seedlings (4 rosette leaves) and snap frozen in liquid nitrogen before RNA preparation.
- 实验种类:
- transcription profiling by array
- 样本量:
- 6
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-20228, GSE20228
- 数据状态:
无法自动分析，您可以尝试手动分析数据。
DataTable pData
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