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题目:: Regulation of Fibroblast Growth Factor-2 by an endogenous antisense RNA and by Argonaute-2
ID:
状态:: 发布时间Feb. 21, 2010 , 更新时间 May 2, 2014 , 提交时间 Jan. 27, 2010,
物种:: Homo sapiens
摘要:: We have previously reported that elevated fibroblast growth factor-2 (FGF-2) expression is associated with tumor recurrence and reduced survival after surgical resection of esophageal cancer, and that these risks are reduced in tumors co-expressing an endogenous antisense (FGF-AS) RNA. In the present study we examined the role of the endogenous FGF-AS transcript in the regulation of FGF-2 expression in the human lung adenocarcinoma cell line, Seg-1. FGF-2 and FGF-AS were temporally and spatially co-localized in the cytoplasm of individual cells, and knock-down of either FGF-2 or FGF-AS by target specific siRNAs resulted in dose-dependent up-regulation of the complementary transcript and its encoded protein product. Using a luciferase reporter system we show that these effects are mediated by interaction of the endogenous antisense RNA with the 3’UTR of the FGF-2 mRNA. Deletion mapping identified a 392 nt sequence in the 5823 nucleotide FGF-2 untranslated tail which is targeted by FGF-AS. siRNA-mediated knockdown of either FGF-AS or FGF-2 significantly increased the stability of the complementary partner mRNA, demonstrating that these mRNAs are mutually regulatory. Knockdown of FGF-AS also resulted in reduced expression of argonaute-2 (AGO-2) and a number of other elements of the endogenous microRNA/RNAi pathways. Conversely, siRNA-mediated knockdown of AGO-2 significantly increased the stability of the FGF-2 mRNA transcript, and the steady-state levels of both FGF-2 mRNA and protein, suggesting a role for AGO-2 in the regulation of FGF-2 expression. Experiment Overall Design: Gene expression in Seg-1 cells transfected with FGF-AS siRNA was compared to gene expression in Seg-1 cells transfected with negative control siRNA using Affymetrix Human Genome U133 Plus 2.0 arrays
实验种类:: transcription profiling by array
样本量:: 2
实验设计:
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数据号:: E-GEOD-20070, GSE20070
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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