Transcription profiling of normal human kidney, liver, spleen and Universal RNA samples at 2 centres (Translational Genomics Research Institute and Childrens National Medical Center)
Normal kidney, liver, spleen, and Universal RNA from Stratagene were expression profiled across five centers (UCLA, Duke, TGen, Children's National Medical Center in Washington, DC, and University of Pennsylvania) using the Affymetrix, spotted Operon, Agilent, and Amersham arrays to identify differences in expression between microarray platforms as well as centers. To compare the four microarray platforms as well as the reproducibility across the centers. There will be expression differences between the five centers. The following array types were profiled at the centers: UCLA: Affymetrix, Amersham, Agilent; TGen: Affymetrix (3000 scanner); Children's: Affymetrix (2500 scanner); Duke: Operon Oligo; University of Penn.: Affymetrix.