实验库 数据相关信息
- 题目:
- Influence of cold shock on total mRNA expression profile during harvesting Thermus thermophilus HB8 cells
- ID:
- 状态:
- 发布时间Oct. 15, 2010 , 更新时间 June 10, 2011 , 提交时间 Jan. 4, 2010,
- 物种:
- Thermus thermophilus HB8
- 摘要:
- In order to investigate the influence of the cold shock during harvesting the cells, we compare the expression profile of mRNA between in T. thermophilus HB8 cells harvested after arrested the growth on ice and in those harvested after addition of cold ethanol. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70°C for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1000 mL of same medium and then cultivated at 70°C for 8 hours (A600 value being ~2.57). For normal cells (harvested after arrested the growth on ice), 15 mL of culture medium was centrifuged 4270G for 5 min at 4°C. For cold-shock avoided cells (harvested after addition of cold ethanol), 15 mL of culture medium was cooled down immediately by addition of the same volume of ethanol which pre-cooled to -80°C, and then centrifuged in same way as normal cells. Then crude RNA was extracted from each sample.
- 实验种类:
- transcription profiling by array
- 样本量:
- 6
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-19723, GSE19723
- 数据状态:
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