实验库 数据相关信息
- 题目:
- Timecourse of gene expression after oprhaning of virgin Solenopsis invicta fire ant queens
- ID:
- 状态:
- 发布时间Jan. 31, 2010 , 更新时间 May 2, 2014 , 提交时间 Jan. 4, 2010,
- 物种:
- Solenopsis invicta
- 摘要:
- In species with social hierarchies, the death of dominant individuals typically upheaves the social hierarchy and provides an opportunity for subordinate individuals to become reproductives. Such a phenomenon occurs in the monogyne form of the fire ant, Solenopsis invicta, where colonies typically contain a single wingless reproductive queen, thousands of workers and hundreds of winged non-reproductive virgin queens. Upon the death of the mother queen, many virgin queens shed their wings and initiate reproductive development instead of departing on a mating flight. Workers progressively execute almost all of them over the following weeks. To identify the molecular changes that occur in virgin queens as they perceive the loss of their mother queen and begin to compete for reproductive dominance, we collected virgin queens before the loss of their mother queen, six hours after orphaning and 24 hours after orphaning. Their RNA was extracted and hybridized against microarrays to examine the expression levels of approximately 10,000 genes. We identified 297 genes that were consistently differentially expressed after orphaning. These include genes that are putatively involved in the signaling and onset of reproductive development, as well as genes underlying major physiological changes in the young queens. 3 samples: 0h, 6h, 24; five queens pooled per samples; each replicate loop of 3 samples was taken from an independent colony; 8 biological replicates (colonies) used. Hybridization according to a Dye-balanced loop design (no technical replication)
- 实验种类:
- transcription profiling by array
- 样本量:
- 48
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-19721, GSE19721
- 数据状态:
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