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题目:: Expression profiling analysis of mouse E10.5 Magoh mutant brain cortices
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状态:: 发布时间April 14, 2010 , 更新时间 June 10, 2011 , 提交时间 Nov. 24, 2009,
物种:: Mus musculus
摘要:: Human brain structure and size requires regulated division of neural stem cells (NSCs). NSCs undergo precise divisions to self-renew and to produce intermediate neural progenitors (INPs) and neurons. The factors that regulate NSC divisions remain poorly understood, as do mechanistic explanations of how aberrant NSC division causes reduced brain size, as seen in microcephaly. Here we demonstrate that Magoh, a component of the core exon junction complex (EJC) that binds spliced RNA, controls cerebral cortical size by regulating NSC division. Magoh haploinsufficiency causes microcephaly due to INP depletion, neuronal apoptosis, and improper mitotic spindle orientation. Defective mitosis underlies these phenotypes as depletion of EJC components disrupts mitotic spindle integrity, chromosome number and genomic stability. We show that an essential function of Magoh is to regulate expression of the human microcephaly protein, LIS1, and that Lis1 addition rescues neurogenesis defects caused by Magoh knockdown, thus providing a genetic explanation for the microcephaly. This study uncovers new requirements for the EJC in brain development, NSC maintenance, mitosis and chromosome stability, thus implicating this complex in the pathogenesis of microcephaly. Mouse embryonic cortices were used for expression analysis 5 biological replicates each of control (C57BL/6) and Magoh mutant brains were analyzed
实验种类:: transcription profiling by array
样本量:: 10
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数据号:: E-GEOD-19168, GSE19168
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