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题目:: Expression profiling of prospero mutant VNC cells in Drosophila embryos
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状态:: 发布时间June 25, 2010 , 更新时间 May 1, 2014 , 提交时间 Sept. 21, 2009,
物种:: Drosophila melanogaster
摘要:: Paper abstract: Stem cells have the remarkable ability to give rise to both self-renewing and differentiating daughter cells. Drosophila neural stem cells segregate cell-fate determinants from the self-renewing cell to the differentiating daughter at each division. Here, we show that one such determinant, the homeodomain transcription factor Prospero, regulates the choice between stem cell self-renewal and differentiation. We have identified the in vivo targets of Prospero throughout the entire genome. We show that Prospero represses genes required for self-renewal, such as stem cell fate genes and cell-cycle genes. Surprisingly, Prospero is also required to activate genes for terminal differentiation. We further show that in the absence of Prospero, differentiating daughters revert to a stem cell-like fate: they express markers of self-renewal, exhibit increased proliferation, and fail to differentiate. These results define a blueprint for the transition from stem cell self-renewal to terminal differentiation. 6 wildtype samples and 6 prospero mutant samples were hybridised to arrays against a commom reference sample (generated from whole embryo cDNA). The prospero samples were indirectly compared to the wildtype samples via the common reference. Half the wildtype and half of the prospero arrays were dye-swapped.
实验种类:: transcription profiling by array
样本量:: 24
实验设计:
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数据号:: E-GEOD-18213, GSE18213
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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