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题目:: Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment
ID:
状态:: 发布时间Aug. 14, 2010 , 更新时间 March 27, 2012
物种:: Glycine soja
摘要:: Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of the saline-alkaline stress transcriptome is most focused on salt (NaCl) stress. Only a little alkaline (NaHCO3) stress transcriptome is limited to one time point after stress. Time-course analysis and comparative investigation on roots in the alkaline stress condition are needed to understand the gene response networks that are subject to alkaline tolerance. We used microarrays to detail the global programme of gene expression underlying NaHCO3 treatment and identified distinct classes of regulated genes during this process. Three week old Glycine soja seedling roots from 3cm root apex were harvested in two independent biological replicates after 0, 0.5, 1, 3, 6, 12 and 24h treatment with 50mmol/L NaHCO3 stress for RNA extraction and hybridization on Affymetrix microarrays. To minimize biological variance, roots from three plants originating from the same experiment, condition and cultivar was pooled.
实验种类:: transcription profiling by array
样本量:: 14
实验设计:
: 无设计数据
数据号:: E-GEOD-17883, GSE17883
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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