| GeneLibs

题目:: Transcription profiling of E. coli O157:H7 when exposed to sodium benzoate
ID:
状态:: 发布时间Oct. 9, 2009 , 更新时间 June 10, 2011 , 提交时间 Aug. 5, 2009,
物种:: Escherichia coli O157:H7 EDL933
摘要:: Sodium benzoate is a widely used food antimicrobial in drinks and fruit juices. A microarray study was conducted to determine the transcriptional response of Escherichia coli O157:H7 to 0.5% (w/v) sodium benzoate. Stationary phase E. coli O157:H7 grown in 150 ml Luria-Bertani broth (LB) was exposed to 0 (control) and 0.5% sodium benzoate. Each treatment was duplicated and sampled at 0 (immediately after exposure), 5, 15, 30, and 60 min. Total RNA was extracted and analyzed with E. coli 2.0 Gene Chips. Experiment Overall Design: Completely randomized design with 0% (control) and 0.5% (w/v) sodium benzoate treated cells were exposed for 0 (immediately after exposure), 5 min, 15 min, 30 min, and 60 min. Each experiment was replicated once and total RNA was extracted and Affymetrix prokaryotic protocol was followed to hybridize cDNA onto E. coli Genome 2.0 Arrays.
实验种类:: transcription profiling by array
样本量:: 20
实验设计:
: 无设计数据
数据号:: E-GEOD-17519, GSE17519
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息