实验库 数据相关信息

题目:
Expression data in WT and Satb1-/- ES cells
ID:
状态:
发布时间Sept. 1, 2009 , 更新时间 June 10, 2011 , 提交时间 Aug. 2, 2009,
物种:
Mus musculus
摘要:
Satb1 and Satb2 are regulators of higher order chromatin in T cells and osteoblasts repectively. We were interested if Satb1 and Satb2 play a role in the regulation of gene expression in ES cells. In this experiment, we compared the transcriptome of wild type and Satb1-/- ES cells. Interestingly Satb1-/- ES cells display an impaired differentiation potential. WT and Satb1-/- ES cells were grown for 3 days in the presence of LIF and selected for Oct4-expression cells by the addition of hygromycin to the culture medium. Previously the cells had been engineered so that they contain a selectable HygroTK reporter in the the Oct4 locus. Total RNA was harvested and used for hybridization.
实验种类:
transcription profiling by array
样本量:
6
实验设计:
无设计数据
数据号:
E-GEOD-17487, GSE17487
数据状态:

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