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题目:: Cardiac and pulmonary responses to diesel engine particulate in Apo E(-/-) mice II
ID:
状态:: 发布时间June 23, 2010 , 更新时间 May 1, 2014 , 提交时间 July 25, 2009,
物种:: Mus musculus
摘要:: Mice deficient in Apolipoprotein E (Apoe E(-/-)) vs wild type mice were exposed to diesel engine particulate by single dose intratracheal instillation. The transcript-level response of the hearts after 24h and of the lungs after 24 h were subsequently analyzed by the 2-color microarray method. Keywords: stress response, disease state analysis Three mice of the Apo E (-/-) strain were exposed to diesel engine particulate from the National Institute of Standards and Technology via intratracheal instillation. Controls consisted of 3 littermates exposed to saline vehicle. Similarly, three wild type mice were similarly exposed to diesel particulate and three to vehicle control. 24 hours after exposure, mice were humanely sacrificed by carbon dioxide asphyxiation and the hearts and lungs were collected and frozen for analysis of total RNA. In each biological replicate, 2 color microarrays were utilized to compare pooled RNA representing all three animals of each strain, and in each condition and in each tissue; each biological replicate was performed with a dye reversal. Biological replicates of the wild type mice responses to diesel engine particulate were performed three times for heart and lung.
实验种类:: transcription profiling by array
样本量:: 40
实验设计:
: 无设计数据
数据号:: E-GEOD-17331, GSE17331
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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