实验库 数据相关信息

题目:
Overexpression of USF1 in HEK293T cells
ID:
状态:
发布时间Sept. 14, 2009 , 更新时间 March 27, 2012
物种:
Homo sapiens
摘要:
Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.
实验种类:
transcription profiling by array
样本量:
6
实验设计:
无设计数据
数据号:
E-GEOD-17300, GSE17300
数据状态:

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