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题目:: Mouse Cells Time of Replication (ToR)
ID:
状态:: 发布时间May 25, 2010 , 更新时间 May 1, 2014
物种:: Mus musculus
摘要:: Measurment of the ToR by comapring the DNA content of the S phase and the DNA content of the G1 phase, the method is based on the method used in the paper - Replication timing of the human genome by Woodfine et al. that was published in Hum. Mol. Genet. 2004. Briefly, after staining with Propidium Iodide, cells in G1 and S phase of the cell cycle are sorted by FACS sorter (ARIA or Vantage), DNA is then extracted from the fractions and differentially labeled with dUTP-Cy3 or dUTP-Cy5 using direct labeling (Agilent labeling kit). The labeled DNA is cohybridized to the array. Early replicating sequences correspond to the spots with high S/G1 ratio and late replicating sequences correspond to the spots with low S/G1 ratio. two mouse tissues were measured (MEF and L1210), 2 replicates for each sample
实验种类:: comparative genomic hybridization by array
样本量:: 8
实验设计:
: 无设计数据
数据号:: E-GEOD-17229, GSE17229
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