发布时间June 24, 2010
, 更新时间 June 10, 2011
, 提交时间 June 16, 2009,
物种:
Saccharomyces cerevisiae
摘要:
Genomic Run-on analysis of topoisomerase mutants (top1-delta/top2ts or top2ts) has been conducted at reference temperature for wt strain and at non-permissive temperature for wt and mutant strains. Keywords: Genomic Run On The analysis includes 3 repeats of the wild type strain (JCW25) at 30ºC and 3 more at 37ºC, 3 repeats of the top2ts mutant (JCW26) at 37ºC and 3 repeats of the double top1 (delta) top2ts mutant at 37ºC. A single genomic DNA hybridization on one of the filters can be used for normalization between gene probes if needed.