Transcription profiling of human BE(2)-C neuronal responses to type I IFN stimulation
ID:
状态:
发布时间July 12, 2009
, 更新时间 June 10, 2011
, 提交时间 June 5, 2009,
物种:
Homo sapiens
摘要:
Human neuronal differentiation alters responsiveness to innate immune stimuli and virus infections. We used microarrays to examine the transcriptional responses of the human BE(2)-C neuroblastoma cell line to retinoic acid-induced differentiation and type I IFN stimulation. Experiment Overall Design: Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks, incubated with universal type I IFN (IFNa-A/D) for 6 or 12 h, and Affymetrix Human Genome Array U133 Plus 2.0 chips were used to analyze transcript levels.