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题目:: Transcription profiling of human HeLa cells treated with MT7
ID:
状态:: 发布时间June 24, 2009 , 更新时间 June 10, 2011 , 提交时间 June 2, 2009,
物种:: Homo sapiens
摘要:: Uncontrolled mitosis forms basis for tumor cell proliferation. Interference with normal mitotic progression often leads tumor cells to mitotic arrest. As a consequence, cell death follows, but not at all time. Reasonably, the antimitotic therapy has been proven effective and indispensable in the clinical treatment of cancer.Recently, we generated a 6H-Pyrido[2',1':2,3]imidazo[4,5-c] isoquinolin -5(6H)-one library, several products of which had been shown to possess antimitotic activities in vitro. In an attempt to understand the antitumor mechanism of these novel chemicals, we have chosen one bioactive product of this library, MT7, as a model compound in this study; We used microarrays to detail the kinetics of gene expression in HeLa cells in a time course of MT7 treatment (from 1.5 h to 12 h). The gene expression profiles were further analyzed by GSEA and Connectivity Map, respectively. Experiment Overall Design: HeLa cells growing on the 10-cm dishes were treated with 5 mM MT7 for 0(control), 1.5(Time1), 3(Time2), 6(Time3) and 12(Time4) hours, respectively. Cells were then washed three times with PBS and the total RNA was isolated and processed for Affymetrix U133A 2.0 hybridizations.
实验种类:: transcription profiling by array
样本量:: 5
实验设计:
: 无设计数据
数据号:: E-GEOD-16378, GSE16378
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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