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题目:: Transcription profiling of mouse NFI-C knock out embryonic fibroblasts
ID:
状态:: 发布时间Nov. 15, 2009 , 更新时间 May 3, 2014 , 提交时间 April 28, 2009,
物种:: Mus musculus
摘要:: The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-β signaling, extracellular matrix deposition and skin appendage pathologies. We performed a global gene expression analysis in NFI-C-/- and wild-type embryonic primary murine fibroblasts. This indicated that NFI-C acts mostly to repress gene expression in response to TGF-β1. Misregulated genes featured a prominent over-representation of regulators of connective tissue inflammation and repair. Experiment Overall Design: mRNAs were isolated from embryonic fibroblasts obtained from 3 wild-type and 3 NFI-C knock-out mice, and their levels were probed using microarrays. Prior to RNA extraction, fibroblast cultures were treated or not with TGF-β1 for 1 hour to examine the immediate response to the growth factor, or treated for 10 hours to assess the delayed response.
实验种类:: transcription profiling by array
样本量:: 18
实验设计:
: 无设计数据
数据号:: E-GEOD-15871, GSE15871
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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