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题目:: shRNA profiling of mouse erythroleukemia (MEL) cells and their corresponding G9a knock downs reveals a dual role for the methyltransferase G9a in the maintenance of beta-globin gene expression
ID:
状态:: 发布时间Nov. 9, 2009 , 更新时间 March 27, 2012 , 提交时间 April 9, 2009,
物种:: Mus musculus
摘要:: MEL clone 745 cells that are blocked at the pro-erythroblast stage serve as a model for terminal erythroid differentiation when treated with DMSO. A stable MEL cell line expressing (Dox)-dependent shRNA sequence targeting different regions of G9a mRNA was established and screened as previously described in (Demers, 2007 PMID17707229). Knock down was induced by adding 5 micrograms/ml final of Dox in the culture medium. For a total of six samples, 10 micro grams of RNA was extracted from each three biological replicates of mouse erythroleukemia (MEL) cells and their corresponding G9a knock downs. The RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) including the on-column DNAse I digestion step. Experiment Overall Design: Mouse erythroleukemia cells: wild type vs G9 knock down. For each microarray hybridization, data analysis was performed using MAS and GCRMA.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-15620, GSE15620
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