Transcription profiling of mouse HSC at various time points after treatment with 5-Fluorouracil
ID:
状态:
发布时间Nov. 15, 2007
, 更新时间 May 1, 2014
, 提交时间 July 12, 2004,
物种:
Mus musculus
摘要:
HSC (Sca+ SP) were isolated from 8-12 week C57B6 mice at various time points after treatment with 5-Fluorouracil. RNA was isolated from 50,000-100,000 FACS sorted cells and subjected to two rounds of T7 based linear amplification using Ambion's Message Amp kit. Two replicates from each time point were analyzed.