| GeneLibs

题目:: ChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions
ID:
状态:: 发布时间Nov. 3, 2009 , 更新时间 May 1, 2014 , 提交时间 April 6, 2009,
物种:: Escherichia coli str. K-12 substr. MG1655
摘要:: We integrated RNAP binding regions (RBRs) and mRNA transcript abundance to determine segments of contiguous transcription originating from promoter regions. To measure RBRs at a genome scale, we employed a ChIP-chip method to E. coli K-12 MG1655 grown in the presence or absence of rifampicin under multiple growth conditions using antibody against E. coli RNAP beta subunit. A twelve ChIP-chip study using immunoprecipitated DNA (IP-DNA) from four separate culture conditions with and/or without rifampicin treatment. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome (NimbleGen). Experiments were conducted as biological duplicates or triplicates (different cultures).
实验种类:: ChIP-chip by tiling array
样本量:: 24
实验设计:
: 无设计数据
数据号:: E-GEOD-15588, GSE15588
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息