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题目:: siRNA of human MCF-7 cells
ID:
状态:: 发布时间Dec. 19, 2009 , 更新时间 May 1, 2014 , 提交时间 March 31, 2009,
物种:: Homo sapiens
摘要:: Overexpression of the AP-2γ transcription factor in breast tumours has been identified as an independent predictor of poor outcome and failure of hormone therapy, even in ER positive, ErbB2 negative tumours; markers of a more favourable prognosis. To understand further the role of AP-2γ in breast carcinoma, we have used an RNA interference and gene expression profiling strategy using the MCF-7 cell line as a model for ER positive, ErbB2 negative tumours with AP-2γ overexpression. Gene expression changes between control and silenced cells implicate AP-2γ in the control of cell cycle progression and developmental signalling. Experiment Overall Design: We compared the expression profiles of MCF-7 cells separately transfected with three independent AP-2γ targeting sequences with those from control cells treated with transfection reagent alone or a non-silencing control siRNA on Affymetrix arrays; each condition was examined in triplicate.
实验种类:: transcription profiling by array
样本量:: 15
实验设计:
: 无设计数据
数据号:: E-GEOD-15481, GSE15481
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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