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题目:: Transcription profiling of human embryonic stem cells reveals conditioned media from SDEC supports proliferation on type I collagen
ID:
状态:: 发布时间Dec. 4, 2009 , 更新时间 June 10, 2011 , 提交时间 March 25, 2009,
物种:: Homo sapiens
摘要:: Conditioned media from a human embryonic germ cell-derived line called SDEC was found to be supportive of human embryonic stem cell growth in the absence of feeder layers on a simple type I collagen matrix. We performed gene expression studies comparing this line to non-supportive cell lines (WI-38 and Detroit 551) to try to identify gene targets responsible for this phenomenon. We used Affymetrix microarrays to identify genes that are differentially regulated in SDEC vs. non-supportive cell lines. The goal is to determine which genes maybe be contributing to human embryonic stem cell growth in the absence of a mouse fibroblast feeder layer. Experiment Overall Design: Total RNA samples were extracted from SDEC, WI-38, and Detroit 551 (D551) cell lines to compare gene expressions. Three biological replicates of each were analyzed via micorarray. Gene targets were identified by looking for highly differentially regulated genes in SDEC compared to the non-supportive lines. We concentrated on secreted proteins (which could potentially be secreted into the conditioned media) which we identified by functional annotations and literature research.
实验种类:: transcription profiling by array
样本量:: 9
实验设计:
: 无设计数据
数据号:: E-GEOD-15400, GSE15400
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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