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题目:: Cell-context dependent Notch target genes
ID:
状态:: 发布时间July 13, 2010 , 更新时间 March 27, 2012
物种:: Mus musculus
摘要:: Notch signaling regulates a variety of developmental cell fates decisions in a cell-context dependent manner. Although Notch signaling directly regulates transcription via the RBP-J/CSL DNA binding protein, little is known about the genes in the respective tissues that are directly activated by Notch. To analyze how Notch signaling mediates its context dependent functions, we utilized a Tamoxifen(OHT)-inducible system (NERT) to activate Notch1 in embryonic stem cells (ESC) at different stages of mesodermal differentiation combined with global transcriptional analyses. Notch1 signaling pathway was analysed in mouse embryonic stem cells (mESCs) that were kept under self-renewal conditions enabling ectodermal, or mesodermal gene expression (ESCe or ESCm respectively), or were differentiated into mesodermal progenitors. NotchIC were induced or not induced by hydroxytamoxifen (OHT) and addititionally in some experiments the cells were treated with protein synthesis inhibitor cycloheximide (CHX) for 4 h.
实验种类:: transcription profiling by array
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-GEOD-15268, GSE15268
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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