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题目:: Effect of s100b on lan5 cells
ID:
状态:: 发布时间June 8, 2010 , 更新时间 May 1, 2014 , 提交时间 March 13, 2009,
物种:: Homo sapiens
摘要:: The human neuroblastoma cell line Lan5 cells were cultured in complete DMEM till 80% confluence, then placed at 60000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 hours prior to s100b treatment. Gene expression patterns were then analysed using the Human Focus Genechip array. In this study we analyze gene expression patterns in Lan5 cells treated with s100b protein. We utilized control cells (untreated) and two different concentrations (0.5 nM and 5 uM) of s100b. We used three biological replicates, for the three concentration tested, according to MIAME guidelines (total 9 chips were used in this study).
实验种类:: transcription profiling by array
样本量:: 9
实验设计:
: 无设计数据
数据号:: E-GEOD-15218, GSE15218
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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