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题目:: Transcription profiling of human induced pluripotent stem cells free of exogenous DNA derived with episomal vectors
ID:
状态:: 发布时间April 4, 2009 , 更新时间 June 10, 2011 , 提交时间 March 10, 2009,
物种:: Homo sapiens
摘要:: Human induced pluripotent stem (iPS) cells have previously been derived from somatic cells using viral vectors that integrate transgenes into the genome. Genomic integration, however, can allow persistent leaky expression of the transgenes and can create insertional mutations, thus limiting the utility of these cells for both research and clinical applications. Here, we describe the derivation of human iPS cells free of vector and transgene sequences using non-integrating oriP/EBNA1-based episomal vectors. The resulting iPS cells are similar to human embryonic stem (ES) cells in both proliferative and developmental potential. These results demonstrate that reprogramming of human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors, and removes one important obstacle to the clinical applications of these cells. Experiment Overall Design: Total of 5 es, 1 fibr, 2 parental, 4 ips sub clones
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-15176, GSE15176
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