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题目:: Transcription profiling of mouse chondrocytes treated with MEK/ERK pathway inhibitors
ID:
状态:: 发布时间May 11, 2009 , 更新时间 May 4, 2014 , 提交时间 March 2, 2009,
物种:: Mus musculus
摘要:: Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation. Experiment Overall Design: Cartilage derived from the hind limbs of CD1 mice staged at 15.5 days of embryonic development were dissected and plated in primary chondrocyte monolayer cultures. Cells were treated with MEK/ERK pathway inhibitors (SB202190 and UO126), a PI3K inhibitor (LY294002), a histone deacetylase inhibitor (Trichostatin A) and the vehicle control (DMSO) for 24 hours. Total RNA was isolated from these samples and hybridized to Affymetrix MOE 430 2.0 chips at the London Regional Genomics facility. Experiment Overall Design: A total of 15 genechips were analyzed between 4 treatments and the vehicle (DMSO) control. Three biological replicates per treatment were executed. Experiment Overall Design: Experiment Overall Design:
实验种类:: transcription profiling by array
样本量:: 15
实验设计:
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数据号:: E-GEOD-15069, GSE15069
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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