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题目:: Transcription profiling of E. coli BW25113 fhlAL/pCA24N-FhlA133 vs fhlA/pCA24N-FhlA in modified-complex 20 mM formate at 37A?C
ID:
状态:: 发布时间Dec. 19, 2009 , 更新时间 May 4, 2014 , 提交时间 Dec. 10, 2008,
物种:: Escherichia coli
摘要:: Variant FhlA133 (Q11H, L14V, Y177F, K245R, M288K, and I342F) had eight- fold higher hydrogen production than FhlA wild-type under 30 min of anaerobic incubation in modified-complex 20 mM formate at 37ºC. The mechanism by which the FhlA133 mutations increase hydrogen production is by increasing the transcription of all of the genes activated by the native FhlA (FHL complex). Experiment Overall Design: Strains: E. coli BW25113 fhlA/pCA24N-FhlA wild-type and E. coli BW25113 fhlA/pCA24N-FhlA133 (Q11H, L14V, Y177F, K245R, M288K, and I342F). Experiment Overall Design: Medium: Modified-complex 20 mM formate Experiment Overall Design: 1 mL of overnight culture in modified - complex 20 mM formate + Cm 30 µg/mL was inoculated in 9 mL of fresh modified - complex 20 mM formate + Cm 30 µg/mL and incubated anaerobically at 37oC in 27 mL glass vials. Experiment Overall Design: Time: 30 minutes Experiment Overall Design: Cell type: Suspension
实验种类:: transcription profiling by array
样本量:: 2
实验设计:
: 无设计数据
数据号:: E-GEOD-13902, GSE13902
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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