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题目:: UV treated Escherichia coli reveals a high number of differentially expressed regions and many novel transcripts
ID:
状态:: 发布时间May 6, 2010 , 更新时间 March 27, 2012 , 提交时间 Dec. 4, 2008,
物种:: Escherichia coli str. K-12 substr. MG1655
摘要:: The transcriptional changes in Escherichia coli upon induction of the SOS response are investigated by utilizing custom designed oligonucleotide microarrays. Keywords: Gene expression during the SOS response in Escherichia coli Escherichia coli K-12 MG1655 single colony in five parallells was grown to mid-log phase and exposed to UV to induce the SOS response. Total RNA was extracted from induced and uninduced cells and cDNA was prepared, fragmented and labelled prior to hybridizing to arrays. The arrays was designed to maximize the genomic coverage whilst simultaneous including only probes estimated to give an approximately uniform binding affinity. Regions coding for non-hypothetical proteins or RNAs where covered less densely than the intergenic parts.
实验种类:: transcription profiling by tiling array
样本量:: 10
实验设计:
: 无设计数据
数据号:: E-GEOD-13829, GSE13829
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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