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题目:: Concurrent Versus Individual Binding of HuR and AUF1 to Common Labile Target mRNAs
ID:
状态:: 发布时间June 9, 2010 , 更新时间 March 27, 2012 , 提交时间 May 3, 2004,
物种:: Homo sapiens
摘要:: RNA was isolated from material that had been immunoprecipitated (IP) from Hela cells using antibodies recognizing RNA-binding proteins HuR or AUF1, as well as using a control IgG1 antibody. RNA was reverse-transcribed in the presence of [alpha-33P]dCTP and the radiolabeled product used to hybridize human cDNA arrays. The experiment was repeated using three independent sample sets. The samples were numbered HuR-1, HuR-2, HuR-3, AUF1-1, AUF1-2, AUF1-3, IgG1-1, IgG1-2, IgG1-3. HuR represents RNA from IP reactions using an anti-HuR antibody, AUF1 represents RNA from IP reactions using an anti-AUF1 antibody and, IgG1 represents RNA from IP reactions using an anti-IgG1 antibody. The numbers 1, 2 and 3 correspond to the three independent experimental datasets. Keywords = RNa-binding protein Keywords = mRNA stability Keywords = exosome Keywords = polysome Keywords = RNA motif Keywords: ordered
实验种类:: transcription profiling by array
样本量:: 9
实验设计:
: 无设计数据
数据号:: E-GEOD-1361, GSE1361
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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