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题目:: RNAi profiling of human MCF-7 cells depleted for SIRT1
ID:
状态:: 发布时间June 8, 2009 , 更新时间 June 10, 2011 , 提交时间 Nov. 3, 2008,
物种:: Homo sapiens
摘要:: SIRT1 is a nuclear NAD+-dependent protein deacetylase. Expression microarray analysis was used to study the effect of SIRT1 knockdown on gene expression in MCF-7 breast cancer cells. Experiment Overall Design: SIRT1 stable knockdown was achieved using two retroviral shRNA constructs. An shRNA directed against Luciferase was used to generate the Luc control cells. Three independent biological replicates with matching Luc controls were analyzed using Affymetrix U133A 2.0 microarrays. Experiment Overall Design: These 6 samples were part of a 15-sample microarray analysis (GSE12971) examining expression regulation by SIRT1, PARP-1, PARG and macroH2A. All 15 samples were included for the following data normalization steps: data sets obtained using the GCOS software were grouped based on date of experiment and adjusted for batch effect using the parametric empirical Bayes method (Combat R); all values < 0.01 were adjusted to 0.01; the data were log2 transformed, median centered for each array, and median centered for each gene.
实验种类:: RNAi profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-13459, GSE13459
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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