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题目:: Transcription profiling of mouse HSCs isolated from wild type and p53 null animals reveals p53 Regulates Hematopoietic Stem Cell Quiescence
ID:
状态:: 发布时间Nov. 15, 2008 , 更新时间 May 1, 2014 , 提交时间 Nov. 3, 2008,
物种:: Mus musculus
摘要:: The importance of the p53 protein in the cellular response to DNA damage is well known, but its function during steady-state hematopoiesis has not been established. We have defined a critical role of p53 in regulating hematopoietic stem cell quiescence, especially in promoting the enhanced quiescence seen in HSCs that lack the MEF/ELF4 transcription factor. Transcription profiling of HSCs isolated from wild type and p53 null mice identified Gfi-1 and Necdin as p53 target genes and using lentiviral vectors to upregulate or knockdown the expression of these genes, we show their importance in regulating HSC quiescence. Establishing the role of p53 (and its target genes) in controlling the cell cycle entry of HSCs may lead to therapeutic strategies capable of eliminating quiescent cancer (stem) cells. Experiment Overall Design: RNAs isolated from wild type, p53 -/- and p53 -/- Mef -/- LSK cells were used in oligonucleotide arrays (Affymetrix)
实验种类:: transcription profiling by array
样本量:: 3
实验设计:
: 无设计数据
数据号:: E-GEOD-13448, GSE13448
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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