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题目:: Transcription profiling of zebrafish pineal gland
ID:
状态:: 发布时间May 11, 2009 , 更新时间 June 10, 2011 , 提交时间 Oct. 14, 2008,
物种:: Danio rerio
摘要:: Microarray data allowed detection of genes that are highly expressed in the pineal gland. Experiment Overall Design: Adult Tg(aanat2:EGFP)Y8 transgenic zebrafish in which EGFP marks the pineal gland were used for RNA extraction and hybridization on Affymetrix microarrays. Fish were anesthetized in 1.5 mM Tricane (Sigma) and sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Since the pineal gland is a clock-containing organ and levels of certain transcripts may vary throughout the circadian cycle, glands were collected throughout the 24-hr cycle at 4 hr intervals. During the 24-hr cycle the fish were either maintained in a 12-h light/12-h dark cycle (LD) or kept in constant darkness (DD). 12 pineal glands were collected and pooled at each time point at each light condition, and total RNA was extracted (RNeasy, Qiagen).
实验种类:: transcription profiling by array
样本量:: 14
实验设计:
: 无设计数据
数据号:: E-GEOD-13196, GSE13196
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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