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题目:: Transcription profiling of mouse B16-F10 melanoma cells transfected with FoxD3
ID:
状态:: 发布时间Oct. 26, 2008 , 更新时间 May 4, 2014 , 提交时间 Oct. 8, 2008,
物种:: Mus musculus
摘要:: We wished to examine the genes regulated by FoxD3 in pigment cells to gain understanding in how FoxD3 represses melanoblast specification in the neural crest. For technical reasons, we could not use neural crest cells, so we used melanoma cells, since they are derived from neural crest cells. To this end, we transfected B16-F10 mouse melanoma cells with constructs expressing FoxD3, or FoxD3-VP16, in which the C-terminal portion of FoxD3 (which contains the transcriptional repression domain) has been replaced by the VP16 transcriptional activation domain. Experiment Overall Design: The base vector used for these studies was pMES, which expresses the gene of interest under control of the chick beta-actin promoter. EGFP is also expressed from the bicistronic mRNA through the use of an IRES. Experiment Overall Design: B16-F10 cells were transfected with either empty pMES, pFoxD3 (which contains FoxD3 inserted into pMES), or pFoxD3-VP16 (similar to pFoxD3, except that the C-terminal portion of FoxD3, which contains the transcriptional repression domain, has been replaced by the transcriptional activation domain of VP16). Experiment Overall Design: 24 hours after transfection, EGFP-positive cells were collected by FACS and those cells were subjected to microarray analysis.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-13129, GSE13129
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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